Abstract
Mycobacteriophages are viruses that infect mycobacteria; little is known about their cis-regulatory transcriptional control elements. In previous work, we identified and characterized a regulatory region (40,670- 40,951 bp) in JacoRen57, an AB cluster mycobacteriophage. The regulatory region appears to play a role in regulating the expression of Gene 50. Utilizing qRT-PCR, we investigated further to see if Gene 50 mRNA is produced during an active infection of Mycobacterium smegmatis by JacoRen57.
Included in
mRNA Production of Gene 50 in JacoRen57
Mycobacteriophages are viruses that infect mycobacteria; little is known about their cis-regulatory transcriptional control elements. In previous work, we identified and characterized a regulatory region (40,670- 40,951 bp) in JacoRen57, an AB cluster mycobacteriophage. The regulatory region appears to play a role in regulating the expression of Gene 50. Utilizing qRT-PCR, we investigated further to see if Gene 50 mRNA is produced during an active infection of Mycobacterium smegmatis by JacoRen57.