Abstract

Our research team, ISLAND CURE, is the team to design and build a biological instrument, optical tweezer (OT), with a limited budget. The optical tweezer is an apparatus that moves micro-objects by using laser light. It allows the user to create and control the pico-newton, which is an essential force to move micro-objects. Our research is to design a target DNA sequence (Fig 4.), which our optical tweezer will control. To apply optical tweezer technology to living organisms, DNA is the essential first step. In this research, our team focus is to calculate the necessary forces to unzip DNA with optical tweezers. Thus, we researched and developed a simple DNA sequence that would not interfere with the force relationship between DNA and laser light. Our DNA sequence is structured by four types of the oligonucleotide, lambda DNA, biotin, and digoxigenin.

Share

COinS
 
Apr 20th, 11:00 AM Apr 20th, 1:00 PM

Undergraduate Studies to Design and Build DNA Structure to be Used by Optical Tweezer

Our research team, ISLAND CURE, is the team to design and build a biological instrument, optical tweezer (OT), with a limited budget. The optical tweezer is an apparatus that moves micro-objects by using laser light. It allows the user to create and control the pico-newton, which is an essential force to move micro-objects. Our research is to design a target DNA sequence (Fig 4.), which our optical tweezer will control. To apply optical tweezer technology to living organisms, DNA is the essential first step. In this research, our team focus is to calculate the necessary forces to unzip DNA with optical tweezers. Thus, we researched and developed a simple DNA sequence that would not interfere with the force relationship between DNA and laser light. Our DNA sequence is structured by four types of the oligonucleotide, lambda DNA, biotin, and digoxigenin.

 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.