Location
Northwestern College, Rowenhorst Student Center
Abstract
We isolated, purified, and characterized four novel Mycobacteriphages: Beelzebub, Raela, RedRaider77, and Antonia from soil samples collected from South Dakota, Colorado, and Nebraska. Raela, Beelzebub, and RedRaider77 are among the first cluster S phages sequenced to date, and Antonia was found to be a member of cluster B1. All four phages are lytic, siphoviridae in morphotype, contain double-stranded DNA genomes, and have long, flexible, non-contractile tails. The genomes of Raela, Beelzebub, and RedRaider77 consist of 65,380 base pairs, 65,529 base pairs, and 64,827 respectively. Antonia’s genome contains 68,573 base pairs. Raela, Beelzebub, and RedRaider77 have a 63.4% GC content, while Antonia has a 66.5% GC content. We assigned functions to 34 of 117 genes in Raela, 36 of 118 in Beelzebub, and 31 of 115 in RedRaider77. The three cluster S phages contain minor tail proteins in the far right arm of the phage, a characteristic unique to cluster S mycobacteriophages. We assigned functions to 30 of 101 of Antonia’s genes and found that its genome resembled other cluster B1 phages. None of the four phages contained tRNA genes and all contained both forward and reverse genes.
Annotation of Four Novel Mycobacteriophages: Beelzebub, Raela, RedRaider77, and Antonia
Northwestern College, Rowenhorst Student Center
We isolated, purified, and characterized four novel Mycobacteriphages: Beelzebub, Raela, RedRaider77, and Antonia from soil samples collected from South Dakota, Colorado, and Nebraska. Raela, Beelzebub, and RedRaider77 are among the first cluster S phages sequenced to date, and Antonia was found to be a member of cluster B1. All four phages are lytic, siphoviridae in morphotype, contain double-stranded DNA genomes, and have long, flexible, non-contractile tails. The genomes of Raela, Beelzebub, and RedRaider77 consist of 65,380 base pairs, 65,529 base pairs, and 64,827 respectively. Antonia’s genome contains 68,573 base pairs. Raela, Beelzebub, and RedRaider77 have a 63.4% GC content, while Antonia has a 66.5% GC content. We assigned functions to 34 of 117 genes in Raela, 36 of 118 in Beelzebub, and 31 of 115 in RedRaider77. The three cluster S phages contain minor tail proteins in the far right arm of the phage, a characteristic unique to cluster S mycobacteriophages. We assigned functions to 30 of 101 of Antonia’s genes and found that its genome resembled other cluster B1 phages. None of the four phages contained tRNA genes and all contained both forward and reverse genes.