Abstract
Calmodulin (CaM) is a small dumbbell-shaped, intermediary protein. CaM binds with several hundred different molecules to help control bodily functions. At Northwestern, we use fluorescently labeled CaM to understand these functions further. The goal of this project is to successfully label CaM protein using the fluorescent dye Alexa Fluor 594. After labeling CaM, we will use buffer exchange chromatography to purify the sample. Following that, we will use three processes to verify the successful labeling of CaM: UV/VIS spectroscopy, mass spectroscopy, and fluorescent microscope imaging. Once labeled and purified, our CaM samples can be used for years to come by Northwestern students and professors to understand the unknown functions of bodily enzymes it interacts with.
Included in
Fluorescent Labeling of Calmodulin for Future Application
Calmodulin (CaM) is a small dumbbell-shaped, intermediary protein. CaM binds with several hundred different molecules to help control bodily functions. At Northwestern, we use fluorescently labeled CaM to understand these functions further. The goal of this project is to successfully label CaM protein using the fluorescent dye Alexa Fluor 594. After labeling CaM, we will use buffer exchange chromatography to purify the sample. Following that, we will use three processes to verify the successful labeling of CaM: UV/VIS spectroscopy, mass spectroscopy, and fluorescent microscope imaging. Once labeled and purified, our CaM samples can be used for years to come by Northwestern students and professors to understand the unknown functions of bodily enzymes it interacts with.