Location
Northwestern College, Rowenhorst Student Center
Abstract
The current study investigated binding characteristics of polyclonal anti-phage antibodies previously produced in our lab and attempted to produce monoclonal antibodies against phage proteins. Western blot analysis was used in order to visualize cross-reactivity between the anti-phage antibodies and protein samples from phages belonging to a variety of clusters. Results indicated a high degree of cross-reactivity between anti-Cborch11 and anti-Roots515 polyclonal antibodies and the sample phage proteins, suggesting these protein structures are highly conserved among phage clusters. Polyethylene glycol fusion was used in order to produce monoclonal antibodies against phage proteins, but have yet to be confirmed, and cell culture is ongoing.
Investigating mycobacteriophage cluster relationships: Characterization of anti-mycobacteriophage antibodies
Northwestern College, Rowenhorst Student Center
The current study investigated binding characteristics of polyclonal anti-phage antibodies previously produced in our lab and attempted to produce monoclonal antibodies against phage proteins. Western blot analysis was used in order to visualize cross-reactivity between the anti-phage antibodies and protein samples from phages belonging to a variety of clusters. Results indicated a high degree of cross-reactivity between anti-Cborch11 and anti-Roots515 polyclonal antibodies and the sample phage proteins, suggesting these protein structures are highly conserved among phage clusters. Polyethylene glycol fusion was used in order to produce monoclonal antibodies against phage proteins, but have yet to be confirmed, and cell culture is ongoing.