Location

Northwestern College, Rowenhorst Student Center

Abstract

The current study investigated binding characteristics of polyclonal anti-phage antibodies previously produced in our lab and attempted to produce monoclonal antibodies against phage proteins. Western blot analysis was used in order to visualize cross-reactivity between the anti-phage antibodies and protein samples from phages belonging to a variety of clusters. Results indicated a high degree of cross-reactivity between anti-Cborch11 and anti-Roots515 polyclonal antibodies and the sample phage proteins, suggesting these protein structures are highly conserved among phage clusters. Polyethylene glycol fusion was used in order to produce monoclonal antibodies against phage proteins, but have yet to be confirmed, and cell culture is ongoing.

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Apr 11th, 12:00 AM Apr 11th, 12:00 AM

Investigating mycobacteriophage cluster relationships: Characterization of anti-mycobacteriophage antibodies

Northwestern College, Rowenhorst Student Center

The current study investigated binding characteristics of polyclonal anti-phage antibodies previously produced in our lab and attempted to produce monoclonal antibodies against phage proteins. Western blot analysis was used in order to visualize cross-reactivity between the anti-phage antibodies and protein samples from phages belonging to a variety of clusters. Results indicated a high degree of cross-reactivity between anti-Cborch11 and anti-Roots515 polyclonal antibodies and the sample phage proteins, suggesting these protein structures are highly conserved among phage clusters. Polyethylene glycol fusion was used in order to produce monoclonal antibodies against phage proteins, but have yet to be confirmed, and cell culture is ongoing.

 

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